Tree Tomato T2-LF-RS

Cyphomandra betacea

Tree Tomato

SS-TRT SeriesTier 1

Target Trait

50K

SHU

Reported target: 50K SHU

Technical Specifications

Genomic Edits

GC Content

(39%)

Construct Length

5,000 bp

Promoter System

EF1α constitutive

Expression System

pLenti-CMV cationic lipid nanoparticle delivery

Host Strain

Cationic lipofection suspension culture LC-50

Pathway Edits

CHS promoter swap for enhanced phenylpropanoid flux
CYP450-B1 integration at native PAL locus
PAL1 cDNA overexpression cassette (35S:PAL1)
4CL2 knockout to redirect coumaroyl-CoA toward capsaicinoid branch

Key Interventions

CS overexpression at 12× native baseline via enhanced 35SPAL1 promoter swap to constitutive CaMV 35S enhancerCYP450-B1 co-factor regeneration via AtCPR overexpression

Application Domains

ResearchPharmaceutical

Intended Use

Enables Tree Tomato cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 50K SHU.

Access

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