Commercial Access Only
Specimen SS-COM-BEY-0295 // BEYOND-PEPPERS
Pepino T5-PR-FD
Solanum muricatum
Pepino
SS-PEP SeriesTier 1
Target Trait
1M
SHU
Reported target: 1M SHU
Technical Specifications
Genomic Edits
9
GC Content
(42%)
Construct Length
6,200 bp
Promoter System
NOS synthetic promoter
Expression System
pKanII PEG-mediated transformation
Host Strain
PEG-mediated protoplast suspension culture
Pathway Edits
- CHS promoter swap for enhanced phenylpropanoid flux
- CYP450-B1 integration at native PAL locus
- PAL1 cDNA overexpression cassette (35S:PAL1)
- 4CL2 knockout to redirect coumaroyl-CoA toward capsaicinoid branch
Key Interventions
CS overexpression at 12× native baseline via enhanced 35SPAL1 promoter swap to constitutive CaMV 35S enhancer
Application Domains
Food ManufacturingAgricultural Biotech
Intended Use
Delivers Pepino-platform capsaicinoids at 1M SHU for scalable food-grade heat ingredient manufacturing.
Access
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