Commercial Access Only
Specimen SS-COM-BEY-0285 // BEYOND-PEPPERS
Pepino T4-PR-FD
Solanum muricatum
Pepino
SS-PEP SeriesTier 1
Target Trait
500K
SHU
Reported target: 500K SHU
Technical Specifications
Genomic Edits
8
GC Content
(41%)
Construct Length
5,800 bp
Promoter System
35S CaMV PEG-optimized
Expression System
pKanII PEG-mediated transformation
Host Strain
PEG-mediated protoplast suspension culture
Pathway Edits
- CHS promoter swap for enhanced phenylpropanoid flux
- CYP450-B1 integration at native PAL locus
- PAL1 cDNA overexpression cassette (35S:PAL1)
Key Interventions
CS overexpression at 12× native baseline via enhanced 35SPAL1 promoter swap to constitutive CaMV 35S enhancerCYP450-B1 co-factor regeneration via AtCPR overexpression
Application Domains
Food ManufacturingAgricultural Biotech
Intended Use
Delivers Pepino-platform capsaicinoids at 500K SHU for scalable food-grade heat ingredient manufacturing.
Access
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