Commercial Access Only
Specimen SS-COM-BEY-0276 // BEYOND-PEPPERS
Pepino T3-PR-RS
Solanum muricatum
Pepino
SS-PEP SeriesTier 1
Target Trait
250K
SHU
Reported target: 250K SHU
Technical Specifications
Genomic Edits
7
GC Content
(40%)
Construct Length
5,400 bp
Promoter System
PetE copper-inducible
Expression System
pKanII PEG-mediated transformation
Host Strain
PEG-mediated protoplast suspension culture
Pathway Edits
- CHS promoter swap for enhanced phenylpropanoid flux
- CYP450-B1 integration at native PAL locus
- PAL1 cDNA overexpression cassette (35S:PAL1)
- 4CL2 knockout to redirect coumaroyl-CoA toward capsaicinoid branch
- CS gene cluster insertion at euchromatic safe-harbor locus
Key Interventions
CS overexpression at 12× native baseline via enhanced 35SPAL1 promoter swap to constitutive CaMV 35S enhancer
Application Domains
ResearchPharmaceutical
Intended Use
Enables Pepino cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 250K SHU.
Access
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