Commercial Access Only
Specimen SS-COM-BEY-0227 // BEYOND-PEPPERS
Tomatillo T3-CR-FD
Physalis philadelphica
Tomatillo
SS-TIL SeriesTier 1
Target Trait
250K
SHU
Reported target: 250K SHU
Technical Specifications
Genomic Edits
7
GC Content
(40%)
Construct Length
5,400 bp
Promoter System
OsU6 + CBE4max-RNP
Expression System
SpCas9-NG base-editor ribonucleoprotein complex
Host Strain
CRISPR-RNP biolistic leaf meristem
Pathway Edits
- CHS promoter swap for enhanced phenylpropanoid flux
- CYP450-B1 integration at native PAL locus
- PAL1 cDNA overexpression cassette (35S:PAL1)
- 4CL2 knockout to redirect coumaroyl-CoA toward capsaicinoid branch
- CS gene cluster insertion at euchromatic safe-harbor locus
Key Interventions
CS overexpression at 12× native baseline via enhanced 35SPAL1 promoter swap to constitutive CaMV 35S enhancer
Application Domains
Food ManufacturingAgricultural Biotech
Intended Use
Delivers Tomatillo-platform capsaicinoids at 250K SHU for scalable food-grade heat ingredient manufacturing.
Access
Request Commercial Access
This construct is available exclusively to qualified institutions. Submit an inquiry to receive technical documentation and licensing terms.
Contact Us →No pricing is listed publicly. We respond to all qualified inquiries within 2 business days.