Commercial Access Only

Specimen SS-COM-BEY-0208 // BEYOND-PEPPERS

Tomatillo T1-CR-RS

Physalis philadelphica

Tomatillo

SS-TIL SeriesTier 1

Target Trait

5K

SHU

Reported target: 5K SHU

Technical Specifications

Genomic Edits

5

GC Content

(38%)

Construct Length

4,600 bp

Promoter System

U6-26 sgRNA + 35S BE4max

Expression System

SpCas9-NG base-editor ribonucleoprotein complex

Host Strain

CRISPR-RNP biolistic leaf meristem

Pathway Edits

  • CHS promoter swap for enhanced phenylpropanoid flux
  • CYP450-B1 integration at native PAL locus
  • PAL1 cDNA overexpression cassette (35S:PAL1)

Key Interventions

CS overexpression at 12× native baseline via enhanced 35SPAL1 promoter swap to constitutive CaMV 35S enhancer

Application Domains

ResearchPharmaceutical

Intended Use

Enables Tomatillo cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 5K SHU.

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