Commercial Access Only

Specimen SS-COM-BEY-0180 // BEYOND-PEPPERS

Ground Cherry T3-LF-RS

Physalis pruinosa

Ground Cherry

SS-GCH SeriesTier 1

Target Trait

250K

SHU

Reported target: 250K SHU

Technical Specifications

Genomic Edits

7

GC Content

(40%)

Construct Length

5,400 bp

Promoter System

SV40 early promoter

Expression System

pLenti-CMV cationic lipid nanoparticle delivery

Host Strain

Cationic lipofection suspension culture LC-50

Pathway Edits

  • CHS promoter swap for enhanced phenylpropanoid flux
  • CYP450-B1 integration at native PAL locus
  • PAL1 cDNA overexpression cassette (35S:PAL1)
  • 4CL2 knockout to redirect coumaroyl-CoA toward capsaicinoid branch
  • CS gene cluster insertion at euchromatic safe-harbor locus

Key Interventions

CS overexpression at 12× native baseline via enhanced 35SPAL1 promoter swap to constitutive CaMV 35S enhancer

Application Domains

ResearchPharmaceutical

Intended Use

Enables Ground Cherry cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 250K SHU.

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