Commercial Access Only

Specimen SS-COM-BEY-0157 // BEYOND-PEPPERS

Ground Cherry T1-CR-FD

Physalis pruinosa

Ground Cherry

SS-GCH SeriesTier 1

Target Trait

5K

SHU

Reported target: 5K SHU

Technical Specifications

Genomic Edits

5

GC Content

(38%)

Construct Length

4,600 bp

Promoter System

U6-26 sgRNA + 35S BE4max

Expression System

SpCas9-NG base-editor ribonucleoprotein complex

Host Strain

CRISPR-RNP biolistic leaf meristem

Pathway Edits

  • CHS promoter swap for enhanced phenylpropanoid flux
  • CYP450-B1 integration at native PAL locus
  • PAL1 cDNA overexpression cassette (35S:PAL1)

Key Interventions

CS overexpression at 12× native baseline via enhanced 35SPAL1 promoter swap to constitutive CaMV 35S enhancer

Application Domains

Food ManufacturingAgricultural Biotech

Intended Use

Delivers Ground Cherry-platform capsaicinoids at 5K SHU for scalable food-grade heat ingredient manufacturing.

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