Commercial Access Only
Specimen SS-COM-BEY-0123 // BEYOND-PEPPERS
Tobacco T3-BL-FD
Nicotiana tabacum
Tobacco
SS-TOB SeriesTier 1
Target Trait
250K
SHU
Reported target: 250K SHU
Technical Specifications
Genomic Edits
7
GC Content
(40%)
Construct Length
5,400 bp
Promoter System
CaMV 35S biolistic
Expression System
pAHC17 biolistic expression cassette
Host Strain
PDS-1000/He leaf-disc explant
Pathway Edits
- CHS promoter swap for enhanced phenylpropanoid flux
- CYP450-B1 integration at native PAL locus
- PAL1 cDNA overexpression cassette (35S:PAL1)
- 4CL2 knockout to redirect coumaroyl-CoA toward capsaicinoid branch
- CS gene cluster insertion at euchromatic safe-harbor locus
Key Interventions
CS overexpression at 12× native baseline via enhanced 35SPAL1 promoter swap to constitutive CaMV 35S enhancer
Application Domains
Food ManufacturingAgricultural Biotech
Intended Use
Delivers Tobacco-platform capsaicinoids at 250K SHU for scalable food-grade heat ingredient manufacturing.
Access
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