Commercial Access Only
Specimen SS-COM-BEY-0018 // BEYOND-PEPPERS
Tomato T2-CR-RS
Solanum lycopersicum
Tomato
SS-TOM SeriesTier 1
Target Trait
50K
SHU
Reported target: 50K SHU
Technical Specifications
Genomic Edits
6
GC Content
(39%)
Construct Length
5,000 bp
Promoter System
AtU6-26 + SpCas9-NG
Expression System
SpCas9-NG base-editor ribonucleoprotein complex
Host Strain
CRISPR-RNP biolistic leaf meristem
Pathway Edits
- CHS promoter swap for enhanced phenylpropanoid flux
- CYP450-B1 integration at native PAL locus
- PAL1 cDNA overexpression cassette (35S:PAL1)
- 4CL2 knockout to redirect coumaroyl-CoA toward capsaicinoid branch
Key Interventions
CS overexpression at 12× native baseline via enhanced 35SPAL1 promoter swap to constitutive CaMV 35S enhancerCYP450-B1 co-factor regeneration via AtCPR overexpression
Application Domains
ResearchPharmaceutical
Intended Use
Enables Tomato cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 50K SHU.
Access
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