Specimen SS-F-UMA-991 // UMAMI · IFI-U
L-Aspartate Umami IV-IV-A
Glycine max
Soybean
Target Trait
2.38×
fold-change
L-aspartate TAS1R1 ligand accumulation index
Technical Specifications
SKU
SS-F-UMA-991
Genomic Edits
5
GC Content
44.8-50.8%
Construct Length
3,271 bp
Promoter System
T7/SP6 cell-free
Expression System
Cell-free T7/SP6 in-vitro
Host Strain
Cell-free
Receptor Target
mGluR1 / TAS1R1-TAS1R3
IFI Score
238
Pathway Edits
- GmAAT2 aspartate aminotransferase isoform 2 overexpression under seed-specific beta-conglycinin promoter for enhanced OAA-to-aspartate transamination in soybean seed
- GmASP4 aspartate 4-hydroxylase RNAi knockdown (60%) to prevent aspartate diversion to beta-amiino acids, preserving free L-aspartate pool as TAS1R1 ligand
- GmASNS asparagine synthetase partial suppression (40%) to reduce glutamine-to-asparagine diversion, freeing aspartate precursor for direct TAS1R1 ligand accumulation
- GmGOT1 glutamate oxaloacetate transaminase isoform 1 overexpression for enhanced aspartate synthesis from OAA across multiple cellular compartments
- GmPEPC phosphoenolpyruvate carboxylase upregulation for OAA production from PEP, expanding the substrate pool feeding AAT2-driven aspartate synthesis
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Soybean-based umami ingredients and plant-based protein hydrolysates where elevated free L-aspartate contributes as a secondary TAS1R1 agonist alongside glutamate, delivering a broader amino acid umami character.
License
$3999 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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