IFI-U · L-Aspartate Umami

Specimen SS-F-UMA-969 // UMAMI · IFI-U

L-Aspartate Umami II-AG-A

Glycine max

Soybean

Tier IIL-Aspartate Umami

Target Trait

1.52×

fold-change

L-aspartate TAS1R1 ligand accumulation index

Technical Specifications

SKU

SS-F-UMA-969

Genomic Edits

4

GC Content

45.2-51.2%

Construct Length

2,855 bp

Promoter System

Tissue-specific 2A11 / CaMV35S

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

152

Pathway Edits

  • GmAAT2 aspartate aminotransferase isoform 2 overexpression under seed-specific beta-conglycinin promoter for enhanced OAA-to-aspartate transamination in soybean seed
  • GmASP4 aspartate 4-hydroxylase RNAi knockdown (60%) to prevent aspartate diversion to beta-amiino acids, preserving free L-aspartate pool as TAS1R1 ligand
  • GmASNS asparagine synthetase partial suppression (40%) to reduce glutamine-to-asparagine diversion, freeing aspartate precursor for direct TAS1R1 ligand accumulation
  • GmGOT1 glutamate oxaloacetate transaminase isoform 1 overexpression for enhanced aspartate synthesis from OAA across multiple cellular compartments

Key Interventions

L-aspartate TAS1R1 ligand accumulation via AAT2/GOT1 upregulation and ASP4/ASNS suppression for soybean seed aspartate enrichmentOAA substrate pool expansion via PEPC upregulation ensuring sufficient precursor for AAT2-driven aspartate synthesis under high demandFree aspartate cytosolic pool accumulation via FAP1 permease for mGluR1-accessible L-aspartate as secondary umami TAS1R1 agonist

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
152
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Soybean-based umami ingredients and plant-based protein hydrolysates where elevated free L-aspartate contributes as a secondary TAS1R1 agonist alongside glutamate, delivering a broader amino acid umami character.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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