IFI-U · L-Aspartate Umami

Specimen SS-F-UMA-967 // UMAMI · IFI-U

L-Aspartate Umami I-IV-A

Glycine max

Soybean

Tier IL-Aspartate Umami

Target Trait

1.1×

fold-change

L-aspartate TAS1R1 ligand accumulation index

Technical Specifications

SKU

SS-F-UMA-967

Genomic Edits

3

GC Content

44.7-50.7%

Construct Length

2,410 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free T7/SP6 in-vitro

Host Strain

Cell-free

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

110

Pathway Edits

  • GmAAT2 aspartate aminotransferase isoform 2 overexpression under seed-specific beta-conglycinin promoter for enhanced OAA-to-aspartate transamination in soybean seed
  • GmASP4 aspartate 4-hydroxylase RNAi knockdown (60%) to prevent aspartate diversion to beta-amiino acids, preserving free L-aspartate pool as TAS1R1 ligand
  • GmASNS asparagine synthetase partial suppression (40%) to reduce glutamine-to-asparagine diversion, freeing aspartate precursor for direct TAS1R1 ligand accumulation

Key Interventions

L-aspartate TAS1R1 ligand accumulation via AAT2/GOT1 upregulation and ASP4/ASNS suppression for soybean seed aspartate enrichmentOAA substrate pool expansion via PEPC upregulation ensuring sufficient precursor for AAT2-driven aspartate synthesis under high demandFree aspartate cytosolic pool accumulation via FAP1 permease for mGluR1-accessible L-aspartate as secondary umami TAS1R1 agonist

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
110
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Soybean-based umami ingredients and plant-based protein hydrolysates where elevated free L-aspartate contributes as a secondary TAS1R1 agonist alongside glutamate, delivering a broader amino acid umami character.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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