Fermented Soy Umami V-IV-A

Technical Specifications

Pathway Edits

• ▸AoALP Aspergillus alkaline protease overexpression under amylase promoter for broad-spectrum substrate protein hydrolysis in soy koji fermentation
• ▸AoNPII neutral protease II isoform overexpression for pH-neutral soy protein hydrolysis releasing glutamate-rich peptide fragments
• ▸AoGAP glyceraldehyde-3-phosphate dehydrogenase promoter-driven glutaminase-A expression for sustained glutamine-to-glutamate conversion in koji
• ▸AoGlutaminase-A glutaminase isoform A overexpression for high-flux glutamine hydrolysis releasing free glutamate from peptide-bound glutamine residues
• ▸AoGGT-1 gamma-glutamyltransferase upregulation for gamma-glutamyl bond hydrolysis in oligopeptides generating free glutamate from peptide pools
• ▸AoGDH2 glutamate dehydrogenase isoform 2 overexpression for reductive amination maintaining free glutamate pool replenishment throughout fermentation
• ▸AoIMPDH inosine monophosphate dehydrogenase upregulation for 5′-IMP co-production alongside free glutamate for synergistic TAS1R1/TAS1R3 activation
• ▸AoNAGK N-acetylglutamate kinase feedback-resistant mutant to prevent glutamate diversion into arginine biosynthesis under high-nitrogen conditions
• ▸AoFAD flavin adenine dinucleotide-dependent oxidase co-factor engineering for enhanced GDH2 catalytic efficiency under aerobic koji fermentation
• ▸AoPro1 proline biosynthesis suppression via P5CS partial RNAi to redirect glutamate-semialdehyde toward free glutamate output
• ▸AoGln1 glutamine synthetase rate-limiting variant introduction to slow glutamate re-amination and preserve free glutamate pool in final product

Key Interventions