IFI-U · Fermented Soy Umami

Specimen SS-F-UMA-942 // UMAMI · IFI-U

Fermented Soy Umami III-PR-B

Aspergillus oryzae

Koji Mold

Tier IIIFermented Soy Umami

Target Trait

4.31×

fold-change

Koji-fermentation analog free glutamate output index

Technical Specifications

SKU

SS-F-UMA-942

Genomic Edits

9

GC Content

47.6-53.6%

Construct Length

4,727 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

431

Pathway Edits

  • AoALP Aspergillus alkaline protease overexpression under amylase promoter for broad-spectrum substrate protein hydrolysis in soy koji fermentation
  • AoNPII neutral protease II isoform overexpression for pH-neutral soy protein hydrolysis releasing glutamate-rich peptide fragments
  • AoGAP glyceraldehyde-3-phosphate dehydrogenase promoter-driven glutaminase-A expression for sustained glutamine-to-glutamate conversion in koji
  • AoGlutaminase-A glutaminase isoform A overexpression for high-flux glutamine hydrolysis releasing free glutamate from peptide-bound glutamine residues
  • AoGGT-1 gamma-glutamyltransferase upregulation for gamma-glutamyl bond hydrolysis in oligopeptides generating free glutamate from peptide pools
  • AoGDH2 glutamate dehydrogenase isoform 2 overexpression for reductive amination maintaining free glutamate pool replenishment throughout fermentation
  • AoIMPDH inosine monophosphate dehydrogenase upregulation for 5′-IMP co-production alongside free glutamate for synergistic TAS1R1/TAS1R3 activation
  • AoNAGK N-acetylglutamate kinase feedback-resistant mutant to prevent glutamate diversion into arginine biosynthesis under high-nitrogen conditions
  • AoFAD flavin adenine dinucleotide-dependent oxidase co-factor engineering for enhanced GDH2 catalytic efficiency under aerobic koji fermentation

Key Interventions

Koji-fermentation analog umami maximization via ALP/NPII/glutaminase-A protease+glutaminase cascade for free glutamate liberation from soy substrateGDH2-driven free glutamate replenishment cycle preventing pool depletion during high-flux protease-glutaminase tandem activityIMP co-production via IMPDH upregulation for synergistic TAS1R1/TAS1R3 nucleotide co-agonism alongside protease-generated free glutamate

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
431
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Fermented soy analogs, natural koji-flavor concentrates, and miso-style umami ingredients where engineered Aspergillus oryzae provides accelerated, amplified fermentation-derived free glutamate and IMP co-agonism.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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