IFI-U · 5-Ribonucleotide

Specimen SS-F-UMA-886 // UMAMI · IFI-U

5-Ribonucleotide I-PR-B

Lentinus edodes

Shiitake

Tier I5-Ribonucleotide

Target Trait

2.07×

fold-change

5′-ribonucleotide panel (IMP/GMP/AMP) accumulation index

Technical Specifications

SKU

SS-F-UMA-886

Genomic Edits

5

GC Content

48.5-54.5%

Construct Length

3,193 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

207

Pathway Edits

  • LeADSS adenylosuccinate synthase overexpression under strong gpd promoter for de-novo IMP elevation feeding all three 5′-ribonucleotide branches
  • LeIMPDH2 inosine monophosphate dehydrogenase isoform 2 upregulation for GMP branch augmentation alongside IMP accumulation
  • LeAK1 adenylate kinase 1 overexpression for AMP/ADP/ATP equilibration, maintaining free AMP concentration as third umami 5′-ribonucleotide co-agonist
  • LeAMPD AMP deaminase partial knockdown (45%) to preserve AMP from conversion to IMP, maintaining all three 5′-nucleotide species simultaneously
  • LeRNase T1 upregulation for endogenous RNA hydrolysis releasing purine nucleotides in post-harvest drying phase of shiitake production

Key Interventions

Broad 5′-ribonucleotide panel engineering (IMP + GMP + AMP) for maximum TAS1R1/TAS1R3 receptor saturation via three-nucleotide co-agonismPost-harvest RNA hydrolysis pathway activation via RNase T1 for natural nucleotide release in dried shiitake as commercial umami ingredientPurine salvage pathway optimization ensuring balanced IMP:GMP:AMP co-accumulation for synergistic mGluR1 receptor activation pattern

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
207
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Premium dried shiitake mushroom powder, high-grade mushroom extracts, and natural umami seasoning applications where the full 5′-ribonucleotide spectrum amplifies perceived umami intensity via three-co-agonist TAS1R1/TAS1R3 saturation.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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