IFI-U · Glutamate Synthase

Specimen SS-F-UMA-872 // UMAMI · IFI-U

Glutamate Synthase IV-IV-B

Solanum lycopersicum

Tomato

Tier IVGlutamate Synthase

Target Trait

3.7×

fold-change

GS-GOGAT cycle glutamate export index

Technical Specifications

SKU

SS-F-UMA-872

Genomic Edits

8

GC Content

46.2-52.2%

Construct Length

4,388 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free T7/SP6 in-vitro

Host Strain

Cell-free

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

370

Pathway Edits

  • SlGOGAT1 NADH-dependent glutamate synthase isoform 1 overexpression under fruit-ripening MADS-box RIN promoter for pericarp-specific GS-GOGAT cycle amplification
  • SlGS2 plastidic glutamine synthetase upregulation via dual 35S promoter for chloroplast glutamine synthetase expansion supporting GOGAT substrate supply
  • SlFd-GOGAT ferredoxin-dependent GOGAT chloroplast isoform overexpression for photosynthetically driven glutamate synthesis complementing NADH-GOGAT
  • SlGDH glutamate dehydrogenase overexpression as overflow safety valve ensuring excess ammonium is channeled into glutamate rather than GABA or free ammonia
  • SlNiR nitrite reductase upregulation for enhanced inorganic nitrogen reduction to ammonium supplying GS-GOGAT cycle input
  • SlNR nitrate reductase isoform 2 overexpression for expanded nitrate-to-nitrite conversion, amplifying nitrogen supply to GS
  • SlGS1;3 cytosolic glutamine synthetase isoform 3 ectopic overexpression in pericarp under 2A11 promoter for localized high-expression GS activity
  • SlSNAT serine:glyoxylate aminotransferase partial suppression to reduce photorespiratory ammonium re-fixation competition with primary GS-GOGAT

Key Interventions

GS-GOGAT cycle optimization via GOGAT1/GS2/Fd-GOGAT triple upregulation for maximum net glutamate export in tomato pericarpNitrogen assimilation amplification through NiR/NR upregulation ensuring substrate supply to GS-GOGAT glutamate synthesis cycleOverflow GDH expression preventing ammonium accumulation and directing excess nitrogen into glutamate mGluR1 agonist pool

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
370
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tomato varieties designed for processing applications requiring high free glutamate from primary nitrogen assimilation engineering. Applicable to paste, puree, and concentrate products where natural umami intensity is a primary quality attribute.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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