IFI-U · Glutamate Synthase

Specimen SS-F-UMA-863 // UMAMI · IFI-U

Glutamate Synthase III-IV-A

Solanum lycopersicum

Tomato

Tier IIIGlutamate Synthase

Target Trait

2.65×

fold-change

GS-GOGAT cycle glutamate export index

Technical Specifications

SKU

SS-F-UMA-863

Genomic Edits

6

GC Content

43.8-49.8%

Construct Length

3,644 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free T7/SP6 in-vitro

Host Strain

Cell-free

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

265

Pathway Edits

  • SlGOGAT1 NADH-dependent glutamate synthase isoform 1 overexpression under fruit-ripening MADS-box RIN promoter for pericarp-specific GS-GOGAT cycle amplification
  • SlGS2 plastidic glutamine synthetase upregulation via dual 35S promoter for chloroplast glutamine synthetase expansion supporting GOGAT substrate supply
  • SlFd-GOGAT ferredoxin-dependent GOGAT chloroplast isoform overexpression for photosynthetically driven glutamate synthesis complementing NADH-GOGAT
  • SlGDH glutamate dehydrogenase overexpression as overflow safety valve ensuring excess ammonium is channeled into glutamate rather than GABA or free ammonia
  • SlNiR nitrite reductase upregulation for enhanced inorganic nitrogen reduction to ammonium supplying GS-GOGAT cycle input
  • SlNR nitrate reductase isoform 2 overexpression for expanded nitrate-to-nitrite conversion, amplifying nitrogen supply to GS

Key Interventions

GS-GOGAT cycle optimization via GOGAT1/GS2/Fd-GOGAT triple upregulation for maximum net glutamate export in tomato pericarpNitrogen assimilation amplification through NiR/NR upregulation ensuring substrate supply to GS-GOGAT glutamate synthesis cycleOverflow GDH expression preventing ammonium accumulation and directing excess nitrogen into glutamate mGluR1 agonist pool

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
265
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tomato varieties designed for processing applications requiring high free glutamate from primary nitrogen assimilation engineering. Applicable to paste, puree, and concentrate products where natural umami intensity is a primary quality attribute.

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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