Specimen SS-F-UMA-831 // UMAMI · IFI-U
Protease-Free Amino Acid IV-IV-A
Glycine max
Soybean
Target Trait
3.55×
fold-change
Protease-driven free amino acid pool index
Technical Specifications
SKU
SS-F-UMA-831
Genomic Edits
8
GC Content
44.8-50.8%
Construct Length
4,291 bp
Promoter System
T7/SP6 cell-free
Expression System
Cell-free T7/SP6 in-vitro
Host Strain
Cell-free
Receptor Target
mGluR1 / TAS1R1-TAS1R3
IFI Score
355
Pathway Edits
- GmPrB proteinase B vacuolar serine protease overexpression under seed-specific promoter for systematic storage protein hydrolysis releasing free glutamate and aspartate
- GmLAP leucine aminopeptidase upregulation for exoprotease cleavage of N-terminal amino acids from protein hydrolysate fragments, amplifying free amino acid yield
- GmCPY carboxypeptidase Y homolog introduction from Saccharomyces for C-terminal amino acid liberation complementing LAP N-terminal hydrolysis
- GmVPE vacuolar processing enzyme isoform 1 overexpression for asparaginyl endopeptidase-driven protein fragmentation at internal Asn/Asp sites
- GmSPI serine protease inhibitor partial knockdown (40%) to relieve endogenous inhibition of introduced protease activities
- GmLTP lipid transfer protein 1 suppression to reduce subcellular membrane barrier limiting protease-substrate contact in protein bodies
- GmPSA proteasome subunit alpha overexpression for enhanced ubiquitin-dependent protein turnover feeding free amino acid pool
- GmNPR non-specific protease R introduction from Bacillus subtilis for broad-spectrum protein hydrolysis under neutral pH conditions
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Soy protein hydrolysate production, natural amino acid seasoning ingredients, and plant-based meat flavor systems where protease-generated free glutamate provides authentic fermentation-equivalent umami intensity.
License
$3999 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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