Protease-Free Amino Acid IV-PR-B

Glycine max

Soybean

Tier IVProtease-Free Amino Acid

Target Trait

4.08×

fold-change

Protease-driven free amino acid pool index

Technical Specifications

SKU

SS-F-UMA-830

Genomic Edits

GC Content

49.3-55.3%

Construct Length

4,654 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

408

Pathway Edits

GmPrB proteinase B vacuolar serine protease overexpression under seed-specific promoter for systematic storage protein hydrolysis releasing free glutamate and aspartate
GmLAP leucine aminopeptidase upregulation for exoprotease cleavage of N-terminal amino acids from protein hydrolysate fragments, amplifying free amino acid yield
GmCPY carboxypeptidase Y homolog introduction from Saccharomyces for C-terminal amino acid liberation complementing LAP N-terminal hydrolysis
GmVPE vacuolar processing enzyme isoform 1 overexpression for asparaginyl endopeptidase-driven protein fragmentation at internal Asn/Asp sites
GmSPI serine protease inhibitor partial knockdown (40%) to relieve endogenous inhibition of introduced protease activities
GmLTP lipid transfer protein 1 suppression to reduce subcellular membrane barrier limiting protease-substrate contact in protein bodies
GmPSA proteasome subunit alpha overexpression for enhanced ubiquitin-dependent protein turnover feeding free amino acid pool
GmNPR non-specific protease R introduction from Bacillus subtilis for broad-spectrum protein hydrolysis under neutral pH conditions
GmFAP1 free amino acid permease 1 overexpression for cytosolic accumulation of liberated glutamate from vacuolar hydrolysis

Key Interventions

Protease cascade engineering for systematic storage protein hydrolysis yielding free glutamate, aspartate, and nucleotide co-agonists in soybean seedExo- and endoprotease co-engineering targeting complete protein fragment hydrolysis for maximum free amino acid liberationAmino acid permease upregulation ensuring liberated free amino acids reach cytosolic compartment for mGluR1-accessible pool formation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

408

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Soy protein hydrolysate production, natural amino acid seasoning ingredients, and plant-based meat flavor systems where protease-generated free glutamate provides authentic fermentation-equivalent umami intensity.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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