IFI-U · Protease-Free Amino Acid

Specimen SS-F-UMA-823 // UMAMI · IFI-U

Protease-Free Amino Acid III-IV-A

Glycine max

Soybean

Tier IIIProtease-Free Amino Acid

Target Trait

2.9×

fold-change

Protease-driven free amino acid pool index

Technical Specifications

SKU

SS-F-UMA-823

Genomic Edits

7

GC Content

43.8-49.8%

Construct Length

3,944 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free T7/SP6 in-vitro

Host Strain

Cell-free

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

290

Pathway Edits

  • GmPrB proteinase B vacuolar serine protease overexpression under seed-specific promoter for systematic storage protein hydrolysis releasing free glutamate and aspartate
  • GmLAP leucine aminopeptidase upregulation for exoprotease cleavage of N-terminal amino acids from protein hydrolysate fragments, amplifying free amino acid yield
  • GmCPY carboxypeptidase Y homolog introduction from Saccharomyces for C-terminal amino acid liberation complementing LAP N-terminal hydrolysis
  • GmVPE vacuolar processing enzyme isoform 1 overexpression for asparaginyl endopeptidase-driven protein fragmentation at internal Asn/Asp sites
  • GmSPI serine protease inhibitor partial knockdown (40%) to relieve endogenous inhibition of introduced protease activities
  • GmLTP lipid transfer protein 1 suppression to reduce subcellular membrane barrier limiting protease-substrate contact in protein bodies
  • GmPSA proteasome subunit alpha overexpression for enhanced ubiquitin-dependent protein turnover feeding free amino acid pool

Key Interventions

Protease cascade engineering for systematic storage protein hydrolysis yielding free glutamate, aspartate, and nucleotide co-agonists in soybean seedExo- and endoprotease co-engineering targeting complete protein fragment hydrolysis for maximum free amino acid liberationAmino acid permease upregulation ensuring liberated free amino acids reach cytosolic compartment for mGluR1-accessible pool formation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
290
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Soy protein hydrolysate production, natural amino acid seasoning ingredients, and plant-based meat flavor systems where protease-generated free glutamate provides authentic fermentation-equivalent umami intensity.

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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