IFI-U · Protease-Free Amino Acid

Specimen SS-F-UMA-802 // UMAMI · IFI-U

Protease-Free Amino Acid I-AG-B

Glycine max

Soybean

Tier IProtease-Free Amino Acid

Target Trait

1.84×

fold-change

Protease-driven free amino acid pool index

Technical Specifications

SKU

SS-F-UMA-802

Genomic Edits

5

GC Content

45.8-51.8%

Construct Length

3,165 bp

Promoter System

Tissue-specific 2A11 / CaMV35S

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

184

Pathway Edits

  • GmPrB proteinase B vacuolar serine protease overexpression under seed-specific promoter for systematic storage protein hydrolysis releasing free glutamate and aspartate
  • GmLAP leucine aminopeptidase upregulation for exoprotease cleavage of N-terminal amino acids from protein hydrolysate fragments, amplifying free amino acid yield
  • GmCPY carboxypeptidase Y homolog introduction from Saccharomyces for C-terminal amino acid liberation complementing LAP N-terminal hydrolysis
  • GmVPE vacuolar processing enzyme isoform 1 overexpression for asparaginyl endopeptidase-driven protein fragmentation at internal Asn/Asp sites
  • GmSPI serine protease inhibitor partial knockdown (40%) to relieve endogenous inhibition of introduced protease activities

Key Interventions

Protease cascade engineering for systematic storage protein hydrolysis yielding free glutamate, aspartate, and nucleotide co-agonists in soybean seedExo- and endoprotease co-engineering targeting complete protein fragment hydrolysis for maximum free amino acid liberationAmino acid permease upregulation ensuring liberated free amino acids reach cytosolic compartment for mGluR1-accessible pool formation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
184
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Soy protein hydrolysate production, natural amino acid seasoning ingredients, and plant-based meat flavor systems where protease-generated free glutamate provides authentic fermentation-equivalent umami intensity.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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