Specimen SS-F-UMA-784 // UMAMI · IFI-U
Tomatine Reduction-Umami III-IV-B
Solanum lycopersicum
Tomato
Target Trait
2.59×
fold-change
Alpha-tomatine reduction / latent umami release index
Technical Specifications
SKU
SS-F-UMA-784
Genomic Edits
6
GC Content
48.9-54.9%
Construct Length
3,501 bp
Promoter System
T7/SP6 cell-free
Expression System
Cell-free T7/SP6 in-vitro
Host Strain
Cell-free
Receptor Target
mGluR1 / TAS1R1-TAS1R3
IFI Score
259
Pathway Edits
- SlGAME1 glycoalkaloid metabolism 1 RNAi knockdown (75%) to suppress alpha-tomatine biosynthesis and reveal latent savory character in ripening fruit
- SlGAME4 steroidal alkaloid gamma-solanine methyltransferase RNAi suppression (60%) to block upper tomatine pathway flux complementing GAME1 knockdown
- SlGDH2 glutamate dehydrogenase isoform 2 upregulation for net free glutamate elevation simultaneously with tomatine removal for compounded umami enhancement
- SlTOMA1 tomatidine acetyltransferase heterologous suppression to prevent tomatidine acetylation and shift any residual alkaloid toward inactive forms
- SlUGT steroidal glycosyltransferase UGT73P2 directed-evolution variant for rapid tomatine glycosylation and sequestration in vacuoles, clearing active pool
- SlGS1 cytosolic glutamine synthetase upregulation for sustained glutamine-to-glutamate cycling complementing GDH2-driven free glutamate increase
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Processing tomatoes and specialty fresh-market varieties where bittering tomatine compounds mask natural umami character. Applicable to tomato paste, sauce, and fresh-cut products targeting enhanced natural savory profiles.
License
$1549 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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