Specimen SS-F-UMA-769 // UMAMI · IFI-U
Tomatine Reduction-Umami II-AG-A
Solanum lycopersicum
Tomato
Target Trait
1.78×
fold-change
Alpha-tomatine reduction / latent umami release index
Technical Specifications
SKU
SS-F-UMA-769
Genomic Edits
4
GC Content
45.2-51.2%
Construct Length
2,855 bp
Promoter System
Tissue-specific 2A11 / CaMV35S
Expression System
Agrobacterium-mediated T-DNA
Host Strain
EHA105
Receptor Target
mGluR1 / TAS1R1-TAS1R3
IFI Score
178
Pathway Edits
- SlGAME1 glycoalkaloid metabolism 1 RNAi knockdown (75%) to suppress alpha-tomatine biosynthesis and reveal latent savory character in ripening fruit
- SlGAME4 steroidal alkaloid gamma-solanine methyltransferase RNAi suppression (60%) to block upper tomatine pathway flux complementing GAME1 knockdown
- SlGDH2 glutamate dehydrogenase isoform 2 upregulation for net free glutamate elevation simultaneously with tomatine removal for compounded umami enhancement
- SlTOMA1 tomatidine acetyltransferase heterologous suppression to prevent tomatidine acetylation and shift any residual alkaloid toward inactive forms
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Processing tomatoes and specialty fresh-market varieties where bittering tomatine compounds mask natural umami character. Applicable to tomato paste, sauce, and fresh-cut products targeting enhanced natural savory profiles.
License
$699 · Tier II License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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