Specimen SS-F-UMA-768 // UMAMI · IFI-U
Tomatine Reduction-Umami I-IV-B
Solanum lycopersicum
Tomato
Target Trait
1.49×
fold-change
Alpha-tomatine reduction / latent umami release index
Technical Specifications
SKU
SS-F-UMA-768
Genomic Edits
4
GC Content
46.0-52.0%
Construct Length
2,807 bp
Promoter System
T7/SP6 cell-free
Expression System
Cell-free T7/SP6 in-vitro
Host Strain
Cell-free
Receptor Target
mGluR1 / TAS1R1-TAS1R3
IFI Score
149
Pathway Edits
- SlGAME1 glycoalkaloid metabolism 1 RNAi knockdown (75%) to suppress alpha-tomatine biosynthesis and reveal latent savory character in ripening fruit
- SlGAME4 steroidal alkaloid gamma-solanine methyltransferase RNAi suppression (60%) to block upper tomatine pathway flux complementing GAME1 knockdown
- SlGDH2 glutamate dehydrogenase isoform 2 upregulation for net free glutamate elevation simultaneously with tomatine removal for compounded umami enhancement
- SlTOMA1 tomatidine acetyltransferase heterologous suppression to prevent tomatidine acetylation and shift any residual alkaloid toward inactive forms
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Processing tomatoes and specialty fresh-market varieties where bittering tomatine compounds mask natural umami character. Applicable to tomato paste, sauce, and fresh-cut products targeting enhanced natural savory profiles.
License
$349 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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