Tomatine Reduction-Umami I-AG-A

Solanum lycopersicum

Tomato

Tier ITomatine Reduction-Umami

Target Trait

1.3×

fold-change

Alpha-tomatine reduction / latent umami release index

Technical Specifications

SKU

SS-F-UMA-761

Genomic Edits

GC Content

44.2-50.2%

Construct Length

2,508 bp

Promoter System

Tissue-specific 2A11 / CaMV35S

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

130

Pathway Edits

SlGAME1 glycoalkaloid metabolism 1 RNAi knockdown (75%) to suppress alpha-tomatine biosynthesis and reveal latent savory character in ripening fruit
SlGAME4 steroidal alkaloid gamma-solanine methyltransferase RNAi suppression (60%) to block upper tomatine pathway flux complementing GAME1 knockdown
SlGDH2 glutamate dehydrogenase isoform 2 upregulation for net free glutamate elevation simultaneously with tomatine removal for compounded umami enhancement

Key Interventions

Alpha-tomatine bittering compound removal via GAME1/GAME4 RNAi for unmasking latent mGluR1 umami signal in tomato fruitGDH2 free glutamate elevation co-engineered with tomatine suppression for compounded umami intensity in finished productLOX1 off-note suppression for clean flavor unmasking after tomatine removal, ensuring revealed umami is not accompanied by aldehyde artifacts

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

130

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Processing tomatoes and specialty fresh-market varieties where bittering tomatine compounds mask natural umami character. Applicable to tomato paste, sauce, and fresh-cut products targeting enhanced natural savory profiles.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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