IFI-U · Kokumi-Umami Bridge

Specimen SS-F-UMA-754 // UMAMI · IFI-U

Kokumi-Umami Bridge V-AG-B

Glycine max

Soybean

Tier VKokumi-Umami Bridge

Target Trait

5.75×

fold-change

CaSR + mGluR1 dual activation index

Technical Specifications

SKU

SS-F-UMA-754

Genomic Edits

11

GC Content

47.5-53.5%

Construct Length

5,553 bp

Promoter System

Tissue-specific 2A11 / CaMV35S

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

575

Pathway Edits

  • GmGCL gamma-glutamylcysteine ligase overexpression under seed-specific promoter for glutathione accumulation as CaSR-activating gamma-glutamyl peptide in soy
  • GmGS glutathione synthase upregulation for complete GSH synthesis from GCL product, enabling triple gamma-glutamyl peptide pool formation
  • GmGDH2 glutamate dehydrogenase isoform 2 overexpression for free glutamate co-accumulation ensuring mGluR1 direct agonism alongside CaSR kokumi activity
  • GmGGT gamma-glutamyltransferase upregulation for gamma-Glu-Val-Gly tripeptide synthesis as potent CaSR agonist from GSH pool
  • GmGLN1 glutamine synthetase cytosolic isoform overexpression for sustained glutamine supply to both GCL and GOGAT pathways
  • GmGSR glutathione reductase upregulation for GSSG recycling maintaining reduced GSH pool available for gamma-glutamyl transfer
  • GmPCS phytochelatin synthase partial suppression to prevent GSH diversion into phytochelatin synthesis during metal stress
  • GmAGS acetylglutamate synthase activity modulation to uncouple arginine biosynthesis from free glutamate consumption
  • GmLAP leucine aminopeptidase suppression for gamma-glutamyl dipeptide preservation through seed maturation processing
  • GmNPR1 non-expressor of PR genes partial knockdown to reduce salicylate-linked glutathione consumption pathways

Key Interventions

CaSR + mGluR1 dual-axis receptor activation via co-engineered glutathione (kokumi) + free glutamate (umami) accumulation in soybeanGamma-Glu-Val-Gly tripeptide synthesis via GGT upregulation for potent CaSR agonism augmenting free glutamate mGluR1 signalingKokumi-umami bridge effect engineering targeting continuous mouthfeel, body, and savory depth beyond standard umami profiles

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
575
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Premium soy-based umami ingredients, high-end plant-based meat flavor systems, and functional food products requiring kokumi-umami bridge character for maximum perceived depth, continuity, and mouthfeel.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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