IFI-U · Kokumi-Umami Bridge

Specimen SS-F-UMA-749 // UMAMI · IFI-U

Kokumi-Umami Bridge IV-PR-A

Glycine max

Soybean

Tier IVKokumi-Umami Bridge

Target Trait

4.25×

fold-change

CaSR + mGluR1 dual activation index

Technical Specifications

SKU

SS-F-UMA-749

Genomic Edits

9

GC Content

43.4-49.4%

Construct Length

4,677 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

425

Pathway Edits

  • GmGCL gamma-glutamylcysteine ligase overexpression under seed-specific promoter for glutathione accumulation as CaSR-activating gamma-glutamyl peptide in soy
  • GmGS glutathione synthase upregulation for complete GSH synthesis from GCL product, enabling triple gamma-glutamyl peptide pool formation
  • GmGDH2 glutamate dehydrogenase isoform 2 overexpression for free glutamate co-accumulation ensuring mGluR1 direct agonism alongside CaSR kokumi activity
  • GmGGT gamma-glutamyltransferase upregulation for gamma-Glu-Val-Gly tripeptide synthesis as potent CaSR agonist from GSH pool
  • GmGLN1 glutamine synthetase cytosolic isoform overexpression for sustained glutamine supply to both GCL and GOGAT pathways
  • GmGSR glutathione reductase upregulation for GSSG recycling maintaining reduced GSH pool available for gamma-glutamyl transfer
  • GmPCS phytochelatin synthase partial suppression to prevent GSH diversion into phytochelatin synthesis during metal stress
  • GmAGS acetylglutamate synthase activity modulation to uncouple arginine biosynthesis from free glutamate consumption
  • GmLAP leucine aminopeptidase suppression for gamma-glutamyl dipeptide preservation through seed maturation processing

Key Interventions

CaSR + mGluR1 dual-axis receptor activation via co-engineered glutathione (kokumi) + free glutamate (umami) accumulation in soybeanGamma-Glu-Val-Gly tripeptide synthesis via GGT upregulation for potent CaSR agonism augmenting free glutamate mGluR1 signalingKokumi-umami bridge effect engineering targeting continuous mouthfeel, body, and savory depth beyond standard umami profiles

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
425
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Premium soy-based umami ingredients, high-end plant-based meat flavor systems, and functional food products requiring kokumi-umami bridge character for maximum perceived depth, continuity, and mouthfeel.

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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