IFI-U · Kokumi-Umami Bridge

Specimen SS-F-UMA-736 // UMAMI · IFI-U

Kokumi-Umami Bridge II-IV-B

Glycine max

Soybean

Tier IIKokumi-Umami Bridge

Target Trait

3.16×

fold-change

CaSR + mGluR1 dual activation index

Technical Specifications

SKU

SS-F-UMA-736

Genomic Edits

7

GC Content

47.3-53.3%

Construct Length

4,034 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free T7/SP6 in-vitro

Host Strain

Cell-free

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

316

Pathway Edits

  • GmGCL gamma-glutamylcysteine ligase overexpression under seed-specific promoter for glutathione accumulation as CaSR-activating gamma-glutamyl peptide in soy
  • GmGS glutathione synthase upregulation for complete GSH synthesis from GCL product, enabling triple gamma-glutamyl peptide pool formation
  • GmGDH2 glutamate dehydrogenase isoform 2 overexpression for free glutamate co-accumulation ensuring mGluR1 direct agonism alongside CaSR kokumi activity
  • GmGGT gamma-glutamyltransferase upregulation for gamma-Glu-Val-Gly tripeptide synthesis as potent CaSR agonist from GSH pool
  • GmGLN1 glutamine synthetase cytosolic isoform overexpression for sustained glutamine supply to both GCL and GOGAT pathways
  • GmGSR glutathione reductase upregulation for GSSG recycling maintaining reduced GSH pool available for gamma-glutamyl transfer
  • GmPCS phytochelatin synthase partial suppression to prevent GSH diversion into phytochelatin synthesis during metal stress

Key Interventions

CaSR + mGluR1 dual-axis receptor activation via co-engineered glutathione (kokumi) + free glutamate (umami) accumulation in soybeanGamma-Glu-Val-Gly tripeptide synthesis via GGT upregulation for potent CaSR agonism augmenting free glutamate mGluR1 signalingKokumi-umami bridge effect engineering targeting continuous mouthfeel, body, and savory depth beyond standard umami profiles

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
316
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Premium soy-based umami ingredients, high-end plant-based meat flavor systems, and functional food products requiring kokumi-umami bridge character for maximum perceived depth, continuity, and mouthfeel.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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