IFI-U · Lactic Acid Umami

Specimen SS-F-UMA-700 // UMAMI · IFI-U

Lactic Acid Umami III-BL-B

Saccharomyces cerevisiae

Yeast

Tier IIILactic Acid Umami

Target Trait

1.9×

fold-change

Lactic acid umami taste potentiation index

Technical Specifications

SKU

SS-F-UMA-700

Genomic Edits

5

GC Content

46.5-52.5%

Construct Length

3,293 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic particle bombardment

Host Strain

Particle Gun

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

190

Pathway Edits

  • ScLDHA L-lactate dehydrogenase A heterologous expression from Lactobacillus helveticus for de-novo lactic acid production in yeast fermentation
  • ScPDC1 pyruvate decarboxylase partial suppression (40%) to redirect pyruvate from ethanol pathway toward lactate via introduced LDHA
  • ScPGK1 phosphoglycerate kinase upregulation for enhanced glycolytic flux increasing pyruvate substrate supply to LDHA
  • ScJEN1 lactate transporter overexpression for lactic acid export and re-import cycling optimizing extracellular lactic acid for food matrix delivery
  • ScMDH3 peroxisomal malate dehydrogenase partial suppression to reduce malate-to-OAA flux, increasing malate-to-lactate conversion indirectly via malic enzyme

Key Interventions

Lactic acid umami potentiation via LDHA introduction and PDC suppression for yeast-derived lactic acid as mGluR1 potentiating organic acidPyruvate-to-lactate flux engineering targeting organic acid-mediated umami persistence in fermented food matricesGlycolytic flux amplification via PGK1 upregulation for sustained lactic acid production throughout yeast fermentation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
190
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Yeast-based fermentation flavor ingredients where lactic acid contributes umami persistence and mouthfeel. Applicable to sourdough flavor concentrates, fermented condiment bases, and natural flavor extracts.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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