IFI-U · Betaine mGluR1

Specimen SS-F-UMA-675 // UMAMI · IFI-U

Betaine mGluR1 V-BL-A

Solanum lycopersicum

Tomato

Tier VBetaine mGluR1

Target Trait

2.5×

fold-change

Glycine betaine mGluR1 activity modulation index

Technical Specifications

SKU

SS-F-UMA-675

Genomic Edits

6

GC Content

43.6-49.6%

Construct Length

3,510 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic particle bombardment

Host Strain

Particle Gun

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

250

Pathway Edits

  • SlBADH betaine aldehyde dehydrogenase overexpression under CaMV35S for glycine betaine accumulation from betaine aldehyde oxidation in tomato leaf and fruit tissue
  • SlCMOB choline monooxygenase B upregulation for enhanced choline-to-betaine aldehyde oxidation, amplifying betaine synthesis flux
  • SlBCSA betaine biosynthesis regulatory gene A overexpression for transcriptional activation of full betaine synthesis pathway
  • SlCMO choline monooxygenase ancestral isoform introduction from Spinacia oleracea for expanded substrate-binding capacity
  • SlCHOX choline oxidase heterologous expression from Arthrobacter globiformis for two-step choline-to-betaine direct oxidation pathway
  • SlVTP vacuolar tonoplast protein upregulation for betaine compartmentalization and osmotic stress relief freeing cellular energy for flavor metabolism

Key Interventions

Glycine betaine mGluR1 activity modulation via BADH/CMOB upregulation for osmolyte-mediated umami character enhancementBetaine accumulation for direct mGluR1 allosteric modulation alongside free glutamate for synergistic IFI-U score contributionBetaine-mediated stress tolerance enhancement enabling flavor-optimal ripening conditions and reduced abiotic stress off-notes

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
250
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tomato products requiring umami roundness enhancement via betaine co-accumulation alongside free glutamate. Applicable to premium fresh-market tomatoes and functional food ingredients.

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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