IFI-U · Betaine mGluR1

Specimen SS-F-UMA-674 // UMAMI · IFI-U

Betaine mGluR1 V-AG-B

Solanum lycopersicum

Tomato

Tier VBetaine mGluR1

Target Trait

2.88×

fold-change

Glycine betaine mGluR1 activity modulation index

Technical Specifications

SKU

SS-F-UMA-674

Genomic Edits

7

GC Content

47.5-53.5%

Construct Length

3,873 bp

Promoter System

Tissue-specific 2A11 / CaMV35S

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

288

Pathway Edits

  • SlBADH betaine aldehyde dehydrogenase overexpression under CaMV35S for glycine betaine accumulation from betaine aldehyde oxidation in tomato leaf and fruit tissue
  • SlCMOB choline monooxygenase B upregulation for enhanced choline-to-betaine aldehyde oxidation, amplifying betaine synthesis flux
  • SlBCSA betaine biosynthesis regulatory gene A overexpression for transcriptional activation of full betaine synthesis pathway
  • SlCMO choline monooxygenase ancestral isoform introduction from Spinacia oleracea for expanded substrate-binding capacity
  • SlCHOX choline oxidase heterologous expression from Arthrobacter globiformis for two-step choline-to-betaine direct oxidation pathway
  • SlVTP vacuolar tonoplast protein upregulation for betaine compartmentalization and osmotic stress relief freeing cellular energy for flavor metabolism

Key Interventions

Glycine betaine mGluR1 activity modulation via BADH/CMOB upregulation for osmolyte-mediated umami character enhancementBetaine accumulation for direct mGluR1 allosteric modulation alongside free glutamate for synergistic IFI-U score contributionBetaine-mediated stress tolerance enhancement enabling flavor-optimal ripening conditions and reduced abiotic stress off-notes

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
288
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tomato products requiring umami roundness enhancement via betaine co-accumulation alongside free glutamate. Applicable to premium fresh-market tomatoes and functional food ingredients.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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