IFI-U · Asparagine-to-Glutamate

Specimen SS-F-UMA-624 // UMAMI · IFI-U

Asparagine-to-Glutamate III-IV-B

Solanum lycopersicum

Tomato

Tier IIIAsparagine-to-Glutamate

Target Trait

2.42×

fold-change

Asparagine-to-glutamate conversion efficiency

Technical Specifications

SKU

SS-F-UMA-624

Genomic Edits

6

GC Content

48.9-54.9%

Construct Length

3,581 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free T7/SP6 in-vitro

Host Strain

Cell-free

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

242

Pathway Edits

  • SlASNS1 asparagine synthetase 1 partial suppression (55% via amiRNA) to reduce glutamine diversion to asparagine, freeing glutamine for GS-GOGAT glutamate cycling
  • SlASP4 aspartate 4-hydroxylase RNAi knockdown to block asparagine catabolism to succinate, redirecting aspartate toward glutamate via AAT2
  • SlGDH glutamate dehydrogenase overexpression for enhanced reductive amination of alpha-ketoglutarate to glutamate under accumulated ammonium from ASNS suppression
  • SlAAT2 aspartate aminotransferase isoform 2 upregulation for enhanced aspartate-to-oxaloacetate transamination freeing glutamate equivalents
  • SlNSP asparagine peptide hydrolase overexpression to liberate free asparagine from peptide pools for downstream ASN-to-GLU conversion
  • SlGS2 plastidic glutamine synthetase upregulation for capturing freed ammonium into glutamine as intermediate for GOGAT-driven glutamate elevation

Key Interventions

ASN-to-GLU pathway re-routing via ASNS1 suppression and GDH/AAT2 upregulation for net free glutamate accumulation in ripening tomatoNitrogen recycling engineering capturing asparagine amide nitrogen into glutamate pool for mGluR1 direct agonismAspartate-to-glutamate carbon routing via AAT2 for complementary free amino acid pool conversion supporting umami intensity

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
242
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Processing tomatoes and tomato-based ingredients where high asparagine content can be redirected to free glutamate during ripening for natural umami enhancement without exogenous additions.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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