Asparagine-to-Glutamate II-PR-A

Solanum lycopersicum

Tomato

Tier IIAsparagine-to-Glutamate

Target Trait

1.65×

fold-change

Asparagine-to-glutamate conversion efficiency

Technical Specifications

SKU

SS-F-UMA-613

Genomic Edits

GC Content

44.6-50.6%

Construct Length

2,803 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

165

Pathway Edits

SlASNS1 asparagine synthetase 1 partial suppression (55% via amiRNA) to reduce glutamine diversion to asparagine, freeing glutamine for GS-GOGAT glutamate cycling
SlASP4 aspartate 4-hydroxylase RNAi knockdown to block asparagine catabolism to succinate, redirecting aspartate toward glutamate via AAT2
SlGDH glutamate dehydrogenase overexpression for enhanced reductive amination of alpha-ketoglutarate to glutamate under accumulated ammonium from ASNS suppression
SlAAT2 aspartate aminotransferase isoform 2 upregulation for enhanced aspartate-to-oxaloacetate transamination freeing glutamate equivalents

Key Interventions

ASN-to-GLU pathway re-routing via ASNS1 suppression and GDH/AAT2 upregulation for net free glutamate accumulation in ripening tomatoNitrogen recycling engineering capturing asparagine amide nitrogen into glutamate pool for mGluR1 direct agonismAspartate-to-glutamate carbon routing via AAT2 for complementary free amino acid pool conversion supporting umami intensity

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

165

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Processing tomatoes and tomato-based ingredients where high asparagine content can be redirected to free glutamate during ripening for natural umami enhancement without exogenous additions.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Umami · IFI-U All Modalities