Asparagine-to-Glutamate I-IV-B

Solanum lycopersicum

Tomato

Tier IAsparagine-to-Glutamate

Target Trait

1.38×

fold-change

Asparagine-to-glutamate conversion efficiency

Technical Specifications

SKU

SS-F-UMA-608

Genomic Edits

GC Content

46.0-52.0%

Construct Length

2,887 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free T7/SP6 in-vitro

Host Strain

Cell-free

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

138

Pathway Edits

SlASNS1 asparagine synthetase 1 partial suppression (55% via amiRNA) to reduce glutamine diversion to asparagine, freeing glutamine for GS-GOGAT glutamate cycling
SlASP4 aspartate 4-hydroxylase RNAi knockdown to block asparagine catabolism to succinate, redirecting aspartate toward glutamate via AAT2
SlGDH glutamate dehydrogenase overexpression for enhanced reductive amination of alpha-ketoglutarate to glutamate under accumulated ammonium from ASNS suppression
SlAAT2 aspartate aminotransferase isoform 2 upregulation for enhanced aspartate-to-oxaloacetate transamination freeing glutamate equivalents

Key Interventions

ASN-to-GLU pathway re-routing via ASNS1 suppression and GDH/AAT2 upregulation for net free glutamate accumulation in ripening tomatoNitrogen recycling engineering capturing asparagine amide nitrogen into glutamate pool for mGluR1 direct agonismAspartate-to-glutamate carbon routing via AAT2 for complementary free amino acid pool conversion supporting umami intensity

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

138

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Processing tomatoes and tomato-based ingredients where high asparagine content can be redirected to free glutamate during ripening for natural umami enhancement without exogenous additions.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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