IFI-U · Glutamyl Transpeptidase

Specimen SS-F-UMA-584 // UMAMI · IFI-U

Glutamyl Transpeptidase III-IV-B

Aspergillus oryzae

Koji Mold

Tier IIIGlutamyl Transpeptidase

Target Trait

2.88×

fold-change

GGT-mediated free glutamate release index

Technical Specifications

SKU

SS-F-UMA-584

Genomic Edits

6

GC Content

48.9-54.9%

Construct Length

3,501 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free T7/SP6 in-vitro

Host Strain

Cell-free

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

288

Pathway Edits

  • AoGGT-1 gamma-glutamyl transpeptidase 1 overexpression under amylase promoter for maximal gamma-glutamyl peptide hydrolysis releasing free glutamate from storage protein pools
  • AoProB proline biosynthesis feedback-resistant variant introduction to redirect glutamate-semialdehyde toward proline-independent glutamate cycling
  • AoGlnA glutamine synthetase partial knockdown (30%) to slow glutamate re-amination to glutamine, preserving free glutamate pool in koji fermentate
  • AoNPII neutral protease II upregulation for enhanced substrate protein hydrolysis exposing gamma-glutamyl bonds to GGT-1 activity
  • AoGAP glyceraldehyde-3-phosphate dehydrogenase promoter-driven GGT expression system for constitutive high-level transpeptidase activity
  • AoGDH2 glutamate dehydrogenase isoform 2 upregulation for net reductive amination maintaining free glutamate pool replenishment after GGT cycling

Key Interventions

GGT-1-mediated free glutamate liberation from peptide pools via constitutive transpeptidase overexpression in Aspergillus oryzae fermentationKoji-fermentation glutamate release amplification targeting mGluR1 direct agonism from peptide-bound glutamate precursorsFree glutamate pool maintenance via GDH2 replenishment cycling to sustain mGluR1 activation throughout fermentation process

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
288
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Koji-based fermentation applications including miso, soy sauce, and shio koji where elevated free glutamate from GGT-mediated peptide hydrolysis drives natural mGluR1-based umami intensity.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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