Glutamyl Transpeptidase I-AG-B

Aspergillus oryzae

Koji Mold

Tier IGlutamyl Transpeptidase

Target Trait

1.61×

fold-change

GGT-mediated free glutamate release index

Technical Specifications

SKU

SS-F-UMA-562

Genomic Edits

GC Content

45.8-51.8%

Construct Length

2,905 bp

Promoter System

Tissue-specific 2A11 / CaMV35S

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

161

Pathway Edits

AoGGT-1 gamma-glutamyl transpeptidase 1 overexpression under amylase promoter for maximal gamma-glutamyl peptide hydrolysis releasing free glutamate from storage protein pools
AoProB proline biosynthesis feedback-resistant variant introduction to redirect glutamate-semialdehyde toward proline-independent glutamate cycling
AoGlnA glutamine synthetase partial knockdown (30%) to slow glutamate re-amination to glutamine, preserving free glutamate pool in koji fermentate
AoNPII neutral protease II upregulation for enhanced substrate protein hydrolysis exposing gamma-glutamyl bonds to GGT-1 activity

Key Interventions

GGT-1-mediated free glutamate liberation from peptide pools via constitutive transpeptidase overexpression in Aspergillus oryzae fermentationKoji-fermentation glutamate release amplification targeting mGluR1 direct agonism from peptide-bound glutamate precursorsFree glutamate pool maintenance via GDH2 replenishment cycling to sustain mGluR1 activation throughout fermentation process

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

161

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Koji-based fermentation applications including miso, soy sauce, and shio koji where elevated free glutamate from GGT-mediated peptide hydrolysis drives natural mGluR1-based umami intensity.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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