Specimen SS-F-UMA-552 // UMAMI · IFI-U
Succinate-Umami IV-IV-B
Saccharomyces cerevisiae
Yeast
Target Trait
2.74×
fold-change
Succinic acid umami enhancement index
Technical Specifications
SKU
SS-F-UMA-552
Genomic Edits
7
GC Content
46.2-52.2%
Construct Length
3,968 bp
Promoter System
T7/SP6 cell-free
Expression System
Cell-free T7/SP6 in-vitro
Host Strain
Cell-free
Receptor Target
mGluR1 / TAS1R1-TAS1R3
IFI Score
274
Pathway Edits
- ScSDH1 succinate dehydrogenase subunit 1 partial knockdown (50%) to reduce succinate oxidation to fumarate, accumulating succinic acid in yeast
- ScSDH2 iron-sulfur subunit 2 activity reduction via weak promoter replacement for SDH complex downregulation
- ScFUM1 fumarase overexpression for futile cycling of fumarate-to-malate, indirectly elevating succinate by reducing fumarate pool pressure
- ScMDH2 malate dehydrogenase cytosolic isoform overexpression for enhanced OAA-to-malate cycling supporting succinate accumulation
- ScICL1 isocitrate lyase overexpression for glyoxylate cycle succinate generation as complementary route to TCA-derived succinate
- ScMLS1 malate synthase upregulation for glyoxylate-to-malate step, allowing net succinate export from glyoxylate shunt
- ScSUC2 sucrose transport upregulation as carbon source enrichment to amplify TCA carbon flux entering succinate node
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Fermented yeast extracts, sake lees (kasu), and fermentation-derived flavor concentrates where succinic acid contributes umami body, persistence, and mouthfeel to savory product matrices.
License
$4049 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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