IFI-U · Succinate-Umami

Specimen SS-F-UMA-541 // UMAMI · IFI-U

Succinate-Umami III-PR-A

Saccharomyces cerevisiae

Yeast

Tier IIISuccinate-Umami

Target Trait

1.95×

fold-change

Succinic acid umami enhancement index

Technical Specifications

SKU

SS-F-UMA-541

Genomic Edits

5

GC Content

45.1-51.1%

Construct Length

3,190 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

195

Pathway Edits

  • ScSDH1 succinate dehydrogenase subunit 1 partial knockdown (50%) to reduce succinate oxidation to fumarate, accumulating succinic acid in yeast
  • ScSDH2 iron-sulfur subunit 2 activity reduction via weak promoter replacement for SDH complex downregulation
  • ScFUM1 fumarase overexpression for futile cycling of fumarate-to-malate, indirectly elevating succinate by reducing fumarate pool pressure
  • ScMDH2 malate dehydrogenase cytosolic isoform overexpression for enhanced OAA-to-malate cycling supporting succinate accumulation
  • ScICL1 isocitrate lyase overexpression for glyoxylate cycle succinate generation as complementary route to TCA-derived succinate

Key Interventions

Succinic acid umami potentiation via SDH complex suppression and glyoxylate cycle activation for net succinate accumulation in yeast fermentateTCA cycle succinate node engineering targeting organic acid-mediated mGluR1 potentiation for umami persistence and mouthfeelFlavour carrier succinic acid co-production alongside free glutamate for synergistic umami enhancement in fermented products

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
195
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Fermented yeast extracts, sake lees (kasu), and fermentation-derived flavor concentrates where succinic acid contributes umami body, persistence, and mouthfeel to savory product matrices.

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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