Specimen SS-F-UMA-538 // UMAMI · IFI-U
Succinate-Umami III-AG-B
Saccharomyces cerevisiae
Yeast
Target Trait
2.24×
fold-change
Succinic acid umami enhancement index
Technical Specifications
SKU
SS-F-UMA-538
Genomic Edits
6
GC Content
48.2-54.2%
Construct Length
3,519 bp
Promoter System
Tissue-specific 2A11 / CaMV35S
Expression System
Agrobacterium-mediated T-DNA
Host Strain
EHA105
Receptor Target
mGluR1 / TAS1R1-TAS1R3
IFI Score
224
Pathway Edits
- ScSDH1 succinate dehydrogenase subunit 1 partial knockdown (50%) to reduce succinate oxidation to fumarate, accumulating succinic acid in yeast
- ScSDH2 iron-sulfur subunit 2 activity reduction via weak promoter replacement for SDH complex downregulation
- ScFUM1 fumarase overexpression for futile cycling of fumarate-to-malate, indirectly elevating succinate by reducing fumarate pool pressure
- ScMDH2 malate dehydrogenase cytosolic isoform overexpression for enhanced OAA-to-malate cycling supporting succinate accumulation
- ScICL1 isocitrate lyase overexpression for glyoxylate cycle succinate generation as complementary route to TCA-derived succinate
- ScMLS1 malate synthase upregulation for glyoxylate-to-malate step, allowing net succinate export from glyoxylate shunt
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Fermented yeast extracts, sake lees (kasu), and fermentation-derived flavor concentrates where succinic acid contributes umami body, persistence, and mouthfeel to savory product matrices.
License
$1549 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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