IFI-U · Succinate-Umami

Specimen SS-F-UMA-529 // UMAMI · IFI-U

Succinate-Umami II-AG-A

Saccharomyces cerevisiae

Yeast

Tier IISuccinate-Umami

Target Trait

1.52×

fold-change

Succinic acid umami enhancement index

Technical Specifications

SKU

SS-F-UMA-529

Genomic Edits

4

GC Content

45.2-51.2%

Construct Length

2,775 bp

Promoter System

Tissue-specific 2A11 / CaMV35S

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

152

Pathway Edits

  • ScSDH1 succinate dehydrogenase subunit 1 partial knockdown (50%) to reduce succinate oxidation to fumarate, accumulating succinic acid in yeast
  • ScSDH2 iron-sulfur subunit 2 activity reduction via weak promoter replacement for SDH complex downregulation
  • ScFUM1 fumarase overexpression for futile cycling of fumarate-to-malate, indirectly elevating succinate by reducing fumarate pool pressure
  • ScMDH2 malate dehydrogenase cytosolic isoform overexpression for enhanced OAA-to-malate cycling supporting succinate accumulation

Key Interventions

Succinic acid umami potentiation via SDH complex suppression and glyoxylate cycle activation for net succinate accumulation in yeast fermentateTCA cycle succinate node engineering targeting organic acid-mediated mGluR1 potentiation for umami persistence and mouthfeelFlavour carrier succinic acid co-production alongside free glutamate for synergistic umami enhancement in fermented products

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
152
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Fermented yeast extracts, sake lees (kasu), and fermentation-derived flavor concentrates where succinic acid contributes umami body, persistence, and mouthfeel to savory product matrices.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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