IFI-U · Succinate-Umami

Specimen SS-F-UMA-525 // UMAMI · IFI-U

Succinate-Umami I-PR-A

Saccharomyces cerevisiae

Yeast

Tier ISuccinate-Umami

Target Trait

1.1×

fold-change

Succinic acid umami enhancement index

Technical Specifications

SKU

SS-F-UMA-525

Genomic Edits

3

GC Content

43.9-49.9%

Construct Length

2,496 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

110

Pathway Edits

  • ScSDH1 succinate dehydrogenase subunit 1 partial knockdown (50%) to reduce succinate oxidation to fumarate, accumulating succinic acid in yeast
  • ScSDH2 iron-sulfur subunit 2 activity reduction via weak promoter replacement for SDH complex downregulation
  • ScFUM1 fumarase overexpression for futile cycling of fumarate-to-malate, indirectly elevating succinate by reducing fumarate pool pressure

Key Interventions

Succinic acid umami potentiation via SDH complex suppression and glyoxylate cycle activation for net succinate accumulation in yeast fermentateTCA cycle succinate node engineering targeting organic acid-mediated mGluR1 potentiation for umami persistence and mouthfeelFlavour carrier succinic acid co-production alongside free glutamate for synergistic umami enhancement in fermented products

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
110
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Fermented yeast extracts, sake lees (kasu), and fermentation-derived flavor concentrates where succinic acid contributes umami body, persistence, and mouthfeel to savory product matrices.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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