IFI-U · Peptide Umami

Specimen SS-F-UMA-512 // UMAMI · IFI-U

Peptide Umami IV-IV-B

Glycine max

Soybean

Tier IVPeptide Umami

Target Trait

3.89×

fold-change

Glutamyl dipeptide accumulation index

Technical Specifications

SKU

SS-F-UMA-512

Genomic Edits

8

GC Content

46.2-52.2%

Construct Length

4,268 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free T7/SP6 in-vitro

Host Strain

Cell-free

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

389

Pathway Edits

  • GmGGT1 gamma-glutamyltransferase 1 overexpression under seed-specific 7S globulin promoter for gamma-glutamyl dipeptide generation from storage protein glutamate
  • GmGGT4 isoform 4 upregulation for cytosolic gamma-glutamyltranspeptidase activity expansion in cotyledon cells
  • GmALT alanine aminotransferase overexpression for enhanced alanine supply as gamma-glutamyl-alanine dipeptide second residue
  • GmAlaAT alanine:pyruvate aminotransferase activity boost for pyruvate-to-alanine flux supporting GGT1-mediated dipeptide synthesis
  • GmLAP leucine aminopeptidase partial suppression to slow gamma-glutamyl dipeptide hydrolysis preserving intact peptide pool
  • GmVPE vacuolar processing enzyme activity modulation for controlled seed protein hydrolysis releasing glutamyl peptide fragments
  • GmPSI protease inhibitor I overexpression to suppress serine proteases that degrade glutamyl dipeptides in mature seed
  • GmGS1a glutamine synthetase cytosolic isoform upregulation for enhanced free glutamate pool feeding GGT-mediated peptide assembly

Key Interventions

Gamma-glutamyl dipeptide accumulation via GGT1/GGT4 upregulation for mGluR1-activating peptide umami effect in soy productsGlutamyl-alanine and glutamyl-valine dipeptide co-accumulation targeting umami roundness and body beyond free glutamateProtease suppression maintaining intact gamma-glutamyl peptide pool through seed maturation and food processing

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
389
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Fermented soy products, miso analogs, and soy protein hydrolysates where gamma-glutamyl peptides provide umami body beyond MSG-equivalent free glutamate. Suitable for clean-label umami seasoning development.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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