Peptide Umami II-IV-A

Glycine max

Soybean

Tier IIPeptide Umami

Target Trait

2.13×

fold-change

Glutamyl dipeptide accumulation index

Technical Specifications

SKU

SS-F-UMA-495

Genomic Edits

GC Content

45.4-51.4%

Construct Length

3,177 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free T7/SP6 in-vitro

Host Strain

Cell-free

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

213

Pathway Edits

GmGGT1 gamma-glutamyltransferase 1 overexpression under seed-specific 7S globulin promoter for gamma-glutamyl dipeptide generation from storage protein glutamate
GmGGT4 isoform 4 upregulation for cytosolic gamma-glutamyltranspeptidase activity expansion in cotyledon cells
GmALT alanine aminotransferase overexpression for enhanced alanine supply as gamma-glutamyl-alanine dipeptide second residue
GmAlaAT alanine:pyruvate aminotransferase activity boost for pyruvate-to-alanine flux supporting GGT1-mediated dipeptide synthesis
GmLAP leucine aminopeptidase partial suppression to slow gamma-glutamyl dipeptide hydrolysis preserving intact peptide pool

Key Interventions

Gamma-glutamyl dipeptide accumulation via GGT1/GGT4 upregulation for mGluR1-activating peptide umami effect in soy productsGlutamyl-alanine and glutamyl-valine dipeptide co-accumulation targeting umami roundness and body beyond free glutamateProtease suppression maintaining intact gamma-glutamyl peptide pool through seed maturation and food processing

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

213

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Fermented soy products, miso analogs, and soy protein hydrolysates where gamma-glutamyl peptides provide umami body beyond MSG-equivalent free glutamate. Suitable for clean-label umami seasoning development.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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