IFI-U · GABA-mGluR1

Specimen SS-F-UMA-474 // UMAMI · IFI-U

GABA-mGluR1 V-AG-B

Solanum lycopersicum

Tomato

Tier VGABA-mGluR1

Target Trait

3.68×

fold-change

GABA mGluR1 allosteric modulation index

Technical Specifications

SKU

SS-F-UMA-474

Genomic Edits

8

GC Content

47.5-53.5%

Construct Length

4,253 bp

Promoter System

Tissue-specific 2A11 / CaMV35S

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

368

Pathway Edits

  • SlGAD1 glutamate decarboxylase isoform 1 overexpression under fruit-ripening promoter for GABA accumulation from free glutamate in ripening tomato pericarp
  • SlGAD2 isoform 2 co-overexpression via bidirectional promoter for expanded GABA biosynthesis capacity across fruit development stages
  • SlGABA-T GABA transaminase RNAi knockdown (70%) to block GABA catabolism to succinate-semialdehyde, preserving GABA pool for mGluR1 modulation
  • SlSSADH succinate-semialdehyde dehydrogenase partial suppression (40%) to slow downstream GABA shunt flux without TCA cycle impairment
  • SlVAT vacuolar amino acid transporter upregulation for GABA vacuolar compartmentalization and controlled release upon maceration
  • SlPrx2 tonoplast H+-pyrophosphatase upregulation for enhanced vacuolar acidification driving GABA accumulation gradient
  • SlGDH2 glutamate dehydrogenase upregulation ensuring free glutamate substrate supply does not limit GAD-driven GABA synthesis

Key Interventions

GABA mGluR1 allosteric modulation via GAD1/GAD2 upregulation and GABA-T suppression for sustained GABA pool in tomato fruitVacuolar GABA compartmentalization via VAT overexpression for processing-triggered release and mGluR1 activation in food matrixGABA-glutamate balance engineering preventing glutamate depletion while maximizing GABA accumulation for dual mGluR1 signaling

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
368
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Functional tomato products targeting GABA-enriched umami profiles for health-conscious consumers. Applicable to tomato-based beverages, reduced-sodium sauces, and nutraceutical flavor systems claiming natural GABA content.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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