Specimen SS-F-UMA-472 // UMAMI · IFI-U
GABA-mGluR1 IV-IV-B
Solanum lycopersicum
Tomato
Target Trait
3.14×
fold-change
GABA mGluR1 allosteric modulation index
Technical Specifications
SKU
SS-F-UMA-472
Genomic Edits
7
GC Content
46.2-52.2%
Construct Length
4,008 bp
Promoter System
T7/SP6 cell-free
Expression System
Cell-free T7/SP6 in-vitro
Host Strain
Cell-free
Receptor Target
mGluR1 / TAS1R1-TAS1R3
IFI Score
314
Pathway Edits
- SlGAD1 glutamate decarboxylase isoform 1 overexpression under fruit-ripening promoter for GABA accumulation from free glutamate in ripening tomato pericarp
- SlGAD2 isoform 2 co-overexpression via bidirectional promoter for expanded GABA biosynthesis capacity across fruit development stages
- SlGABA-T GABA transaminase RNAi knockdown (70%) to block GABA catabolism to succinate-semialdehyde, preserving GABA pool for mGluR1 modulation
- SlSSADH succinate-semialdehyde dehydrogenase partial suppression (40%) to slow downstream GABA shunt flux without TCA cycle impairment
- SlVAT vacuolar amino acid transporter upregulation for GABA vacuolar compartmentalization and controlled release upon maceration
- SlPrx2 tonoplast H+-pyrophosphatase upregulation for enhanced vacuolar acidification driving GABA accumulation gradient
- SlGDH2 glutamate dehydrogenase upregulation ensuring free glutamate substrate supply does not limit GAD-driven GABA synthesis
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Functional tomato products targeting GABA-enriched umami profiles for health-conscious consumers. Applicable to tomato-based beverages, reduced-sodium sauces, and nutraceutical flavor systems claiming natural GABA content.
License
$4049 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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