IFI-U · Glutathione-Umami

Specimen SS-F-UMA-428 // UMAMI · IFI-U

Glutathione-Umami IV-BL-B

Saccharomyces cerevisiae

Yeast

Tier IVGlutathione-Umami

Target Trait

3.43×

fold-change

Glutathione (gamma-glutamyl peptide) accumulation index

Technical Specifications

SKU

SS-F-UMA-428

Genomic Edits

8

GC Content

47.0-53.0%

Construct Length

4,240 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic particle bombardment

Host Strain

Particle Gun

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

343

Pathway Edits

  • ScGCL1 gamma-glutamylcysteine ligase overexpression under PGK1 promoter for enhanced glutamate-to-gamma-glutamylcysteine ligation in yeast
  • ScGS glutathione synthase upregulation via synthetic TDH3 promoter for complete glutathione tripeptide synthesis from gamma-GC intermediate
  • ScGSR glutathione reductase overexpression to recycle GSSG to GSH, maintaining high reduced glutathione pool for mGluR1-modulating kokumi-umami activity
  • ScGLR1 glutaredoxin 1 activity upregulation for cytosolic redox buffering, preventing GSH oxidation and preserving gamma-glutamyl peptide pool
  • ScGPX2 glutathione peroxidase redirection variant for preferential GSSG reduction over peroxide scavenging, maximizing free GSH
  • ScGCT gamma-glutamyltranspeptidase partial knockdown to prevent GSH hydrolysis and maintain intact gamma-glutamyl dipeptide pool
  • ScMET4 methionine regulon transcription factor activation for enhanced cysteine supply feeding GCL-dependent GSH biosynthesis
  • ScMUP1 methionine permease upregulation for enhanced cysteine precursor uptake from fermentation medium

Key Interventions

Glutathione CaSR + mGluR1 dual-activation engineering via elevated gamma-glutamyl peptide pool for kokumi-umami bridging activityGCL/GS pathway amplification for GSH accumulation targeting gamma-glutamyl peptide-mediated umami roundness and bodyGSH pool oxidation prevention via GSR/GLR1 redox engineering for sustained kokumi-umami activity through fermentation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
343
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Baker's yeast extracts, brewer's yeast concentrates, and fermentation-derived umami ingredients where glutathione delivers kokumi-umami bridging character. Applicable to natural flavor development for processed food manufacturers.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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