Glutathione-Umami II-AG-A

Saccharomyces cerevisiae

Yeast

Tier IIGlutathione-Umami

Target Trait

1.92×

fold-change

Glutathione (gamma-glutamyl peptide) accumulation index

Technical Specifications

SKU

SS-F-UMA-409

Genomic Edits

GC Content

45.2-51.2%

Construct Length

2,735 bp

Promoter System

Tissue-specific 2A11 / CaMV35S

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

192

Pathway Edits

ScGCL1 gamma-glutamylcysteine ligase overexpression under PGK1 promoter for enhanced glutamate-to-gamma-glutamylcysteine ligation in yeast
ScGS glutathione synthase upregulation via synthetic TDH3 promoter for complete glutathione tripeptide synthesis from gamma-GC intermediate
ScGSR glutathione reductase overexpression to recycle GSSG to GSH, maintaining high reduced glutathione pool for mGluR1-modulating kokumi-umami activity
ScGLR1 glutaredoxin 1 activity upregulation for cytosolic redox buffering, preventing GSH oxidation and preserving gamma-glutamyl peptide pool

Key Interventions

Glutathione CaSR + mGluR1 dual-activation engineering via elevated gamma-glutamyl peptide pool for kokumi-umami bridging activityGCL/GS pathway amplification for GSH accumulation targeting gamma-glutamyl peptide-mediated umami roundness and bodyGSH pool oxidation prevention via GSR/GLR1 redox engineering for sustained kokumi-umami activity through fermentation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

192

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Baker's yeast extracts, brewer's yeast concentrates, and fermentation-derived umami ingredients where glutathione delivers kokumi-umami bridging character. Applicable to natural flavor development for processed food manufacturers.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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