IFI-U · Theanine-mGluR1

Specimen SS-F-UMA-392 // UMAMI · IFI-U

Theanine-mGluR1 IV-IV-B

Camellia sinensis

Tea

Tier IVTheanine-mGluR1

Target Trait

2.93×

fold-change

L-theanine mGluR1 potentiation index

Technical Specifications

SKU

SS-F-UMA-392

Genomic Edits

7

GC Content

46.2-52.2%

Construct Length

4,048 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free T7/SP6 in-vitro

Host Strain

Cell-free

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

293

Pathway Edits

  • CsCSHD cystathionine gamma-synthase homolog overexpression for enhanced L-theanine biosynthesis via glutamate-ethylamine condensation in young tea leaves
  • CsGDH glutamate dehydrogenase upregulation under CaMV35S for increased glutamate substrate availability feeding theanine synthase reaction
  • CsTSII theanine synthase isoform II overexpression for expanded glutamate-to-theanine conversion capacity in mesophyll cells
  • CsTDH theanine demethylase activity suppression via RNAi to prevent L-theanine catabolism to glutamate + ethylamine
  • CsATP-binding cassette transporter upregulation for vacuolar L-theanine accumulation and controlled release during tea processing
  • CsGS plastidic isoform overexpression to maintain glutamine pool as indirect theanine precursor without depleting free glutamate
  • CsGOGAT Fd-dependent isoform upregulation for chloroplast-localized glutamate synthesis complementing cytosolic theanine pathway

Key Interventions

L-theanine mGluR1 positive allosteric modulation engineering via CSDH/TSII dual upregulation for elevated tea umami characterGlutamate substrate pool maintenance ensuring theanine synthesis does not deplete free glutamate mGluR1 agonist poolmGluR1 PAM (positive allosteric modulator) concentration maximization in young leaf tissue for premium green tea umami intensity

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
293
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Premium green tea, matcha, and tea extracts where elevated L-theanine drives mGluR1-mediated umami and kokumi-adjacent characters. Ideal for specialty tea producers and functional beverage ingredient suppliers.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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