Free Glutamate Accumulation V-PR-B

Technical Specifications

Pathway Edits

• ▸SlGDH2 glutamate dehydrogenase isoform 2 overexpression under fruit-ripening RIN promoter for TCA-to-glutamate reductive amination burst at red stage
• ▸SlGS1 cytosolic glutamine synthetase feedback-resistant mutant (G295A) for unregulated glutamine-to-glutamate cycling under high-nitrogen conditions
• ▸SlGOGAT glutamine:2-oxoglutarate aminotransferase upregulation for expanded GS-GOGAT cycle flux amplifying net free glutamate output
• ▸SlGLN1;2 pericarp-specific glutamine synthetase overexpression for localized free amino acid accumulation in fruit skin
• ▸SlAAT aspartate aminotransferase isoform 1 redirected via active-site mutation (K256R) favoring glutamate over aspartate output
• ▸SlVGT1 vacuolar glutamate transporter overexpression for apoplastic glutamate accumulation and controlled release upon maceration
• ▸SlGABA-T GABA transaminase partial suppression (50% via miRNA) to reduce GABA shunt diversion from free glutamate pool
• ▸SlNAD-ME NADP-dependent malic enzyme upregulation for enhanced malate-to-OAA conversion feeding alpha-ketoglutarate-to-glutamate pathway
• ▸SlPGSHD phosphoserine aminotransferase knockdown to prevent serine biosynthesis from diverting 3-phosphoglycerate away from TCA/glutamate

Key Interventions